The digestive system of Daphnia is quite active, and the students often think that the moving digestive system is the heart. a. We'll be putting the independent variable as. For ten points, the following two items, and only these items, are due one week from the performance of the experiment: Table of results and Graph: [pic] [pic] Discussion: The more concentrated caffeine solution given to the daphnia, the higher their heart rate became, for example the 0% caffeine solution gave a heart rate of 249 bpm and the 0. Investigating factors affecting the heart rate of Daphnia. Measuring the Heart Rate of Daphnia Daphnia is the name of a group of small, aquatic crustaceans commonly called 'water flies'. This is a logarithmic scale as concentration increases by a factor of 10 each time. I believe that a Daphnia subjected to a caffeine solution will show a rise in heart rate, this rise in heart rate should reflect the concentration of caffeine in a given solution. Caffeine's average percent change was 4.7955 percent in BPM. Daphnia. Explain why you think that the results shown in your graph support or refute your hypothesis Daphnia. Caffeine is a stimulant, which, in humans, causes heart rates and blood pressure to increase. [1]However, some changes that Showing that in trial #2 the control group experienced a larger heartbeat rate decrease than in trial #1. Repeat this for measurements across 5 different caffeine concentrations (e.g. Explain whether the . We'll be putting the independent variable as. To test this hypothesis two Daphnia Magna were exposed to either alcohol or caffeine and its heart rate recorded by placing it on a microscope slide and counting the number of heart beats. When finished the Daphnia was placed into a new beaker of water. 4. 4. The biggest problem with this lab is helping the student to find the heart. Repeat this four times to get and average heart rate. Daphnia. A live Daphnia in a depression slide was examined at room temperature to determine its heart rate. When we added ethanol the daphnia, the heart rate was 157 beats per minute. Get a second Daphnia slide and measure the basal heart rate. Daphnia. - Water from Daphnia extraction may have diluted the caffeine concentration. Figure 1. Table 2: Group data for alcohol concentrations. Determine the effect of temperature on the heart rate. Higher concentrations produced varied and Graph the Substances vs the Average % Change in Heart Rate after deciding: if you should use bar or line graph, which variable should be labeled X axis and Y axis using the data below: . This is what we predicted to happen in our hypothesis. When we added caffeine the daphnia, the heartbeat raised to 161 beats per minute. The small heart of Daphnia is easily visible under the low power of a microscope. 5% solution gave 328 bpm showing an increase. Pour in some water to create a shallow ice bath. The Effects of Caffeine on the Heart Rate of Daphnia This lab was designed to show how caffeine affected the heart rate of daphnia after exposing them to different concentrations of caffeine for ten minutes. Initial heart rate was determined as the average of the first three heart rate determinations for each animal. ← Also, be careful not to spill any of the . Arrows mark the replacement of the bath with the test substance at 30 min and its removal 30 min . 3. View Lab Report - Daphnia lab from BIO Lab at University of Rhode Island. Add a single drop of the selected drug. Identify what your independent and dependent variables are. A cold-blooded animal like Daphnia is directly influenced by temperature. 2. Graphing Data Using Microsoft Excel 2010 1. - Different Daphnia showed different results due to genetic variation. 5% solution gave 328 bpm showing an increase. The hypothesis that ethanol would slow the heart rate of the Daphnia was shown to be accurate. Table of results and Graph: [pic] [pic] Discussion: The more concentrated caffeine solution given to the daphnia, the higher their heart rate became, for example the 0% caffeine solution gave a heart rate of 249 bpm and the 0. We referred to the Daphnia Heart Rate Procedure (Shaw and French, 2018) to see how to properly record the Daphnia's heart rate. One rule states that metabolic rate doubles for every 10 degrees increase in . Results The results of the experiment show a greater heart rate for the daphnia in the alcohol solution. GRAPH OF DAPHNIA UNDER COLD CONDITIONS 2 4 6 8 10 12 10 20 30 40 50 60 70 00 95. (Caffeine increases the heart rate of Daphnia). - place 1 daphnia into cavity slide - remove pond water and replace with distilled water - leave for 5 mins to acclimatise, observe and count heart rate under microscope for 30s, multiply by 2 to calculate bpm - repeat with 2 more daphnia - repeat again with small conc of caffeine solution in place of distilled water - carry out for 5 conc of . 6. Determine and record the heart rate in beats per minute (bpm). 2) Graphs: The graph below shows the Graph of Heart Rate of Daphnia Against Concentration of Caffeine Solution. Repeat all the above steps for your 0.1% solution, and again with your control . The average heart rate of Daphnia under normal condition which acts as a control experiment is 114 heart beats per minute. 00 95. Basal Heart Rate of Daphnia #2 Item beats in 10 seconds (b/10") beats / minute (bpm) Count 1 Count 2 Graph 3: Displaying the heartbeat rate decreases in Daphnia magna within 1 hour of drug exposure for trial #1 and within 48 hours for trial #2. Blood cells are easily visible through the transparent body as they flow rapidly through the body cavity. to the recovery tank. - Left for too long under microscope, temp increased (due to lamp) = increased heart rate. For example: As you see figure 1 by increasing Alcohol concentrations, Daphnia's heart rate decrease. Lab report of Daphnia magna heart rate. . 5. As seen on my graph, the control group of daphnia that was not exposed to any levels of caffeine had an average heart rate of 195.9 beats per minute, the group that was exposed to the .5% concentration of caffeine had an average heart rate of 185.5 beats per minute, and the group that was exposed to the 1% concentration of caffeine had an . Here is a chart of my control: Heart-rate of Daphnia (Control) TRIAL # I II III IV V 1 240 240 248 242 248 2 259 244 243 251 242 BEATS 3 243 237 241 235 251 4 253 246 241 248 243 5 242 247 247 252 249 PER 6 260 241 246 241 241 7 251 248 243 240 239 8 239 240 249 248 246 MINUTE 9 259 243 245 249 252 10 246 249 249 245 237 AVG. Temperature of the Daphnia. Add a drop of caffeine solution to the Daphnia. 5 Results It was observed that the heart rate of Daphnia magna increased with increasing temperature as shown on table 1.0 and Figure 1. the x-axis, so in the Column 1. In the water flea Daphnia, the single, small heart is easily visible when viewed under transmitted light under a low power microscope.The heart rate (which can be up to 300 beats per minute) can be monitored and counted in different conditions - for example changing water temperature, or changing the type and concentration of chemicals added to the water. The y-axis shows values from 0 to 221.05263157894737. Daphnias affected Heart Rate This lab experiment was conducted on Daphnias to test the chemical effects on its heart rate. The hypothesis is accepted. information and graphs reported in the article, follow connections to earlier articles about the effects of . DATA: Put on depression slide. Seventy-two Daphnia were used in the initial experiments. The heart is located dorsally and anterior from the brood chamber.At 20ºC, it beats about 200 times per minute, slowing down at lower temperatures. If the Daphnia is submerged in alcohol the heart rate will slow down, as if it's submerged in . Page 1 of 2. Figure 1: the graph that shows the group average heart rates when exposed to the different concentrations of caffeine provided that the Daphnia Magna heart rate was counted for 15 seconds then calculated to BPM. Effects of Substances on the Heart Rate of Daphnia Magna. The . Temperature of the Daphnia. Use a calculator to calculate the heart rate. First, we tested water and followed by testing two concentrations of caffeine. We will call this rate our BASELINE or CONTROL heartrate. Report. 25. 0.2M, 0.3M, 0.4M and 0.5M). Repeat steps 1-5 with a new . Effect of isopropyl alcohol (A) and club soda (B) on the heart rate of Daphnia magna. Pasteur pipette (for water from the Daphnia culture tank) Concentration of caffeine given to individual daphnia; 0, 0.001, 0.01, 0.1, 1, 10, 100 (%). Global warming is mostly associated with increased extreme temperature . Multiply by four to get the beats per minute. Record your quantitative and qualitative . Answer: the heart rate would slow down considerably. Wait one minute for the solution to be absorbed, and compute the average heart rate as performed above. A mobile phone can be used to video the heart beat. The dependent variable will be the y-axis, so in Column 2. 00 96. the x-axis, so in the Column 1. 2. Results: As you see in the chart below, the daphnia average heart rate is 140 beats per minute. These phenomena may occur even in response to small dose of ethanol exposure or during its withdrawal. Determine the Q10 for the Daphnia heart. Analysis- Part 3 of Rubric 3. 2. Add two or three drops of your 1% solution. The biggest problem with this lab is helping the student to find the heart. The heart beats rise to 153 in solution containing 0.30% caffeine solution. Ask students if the graphs show a similar pattern. Fill the bottom of the large beaker with enough ice to make a layer of ice one to two cubes thick. Enter this information into the data table. Repeat this procedure 2 more times and find the average heart rate of Daphnia. Students may extrapolate from the graph that the heart rate will slow considerably, even assigning a numerical value. A graph was also used to provide a visual representation of the effect of temperature change on the heart rate of Daphnia magna in our experiment. One student will use a stopwatch to count 30 seconds which is the time during which the measurement of the heart rate will take place. If you need assistance building a graph, there is a Guide to Graphing resource available on your Moodle. (See resources above) 3. This is what we predicted to happen in our hypothesis. When repeating a test a different Daphnia is used. (c) Explain that temperature has a general affect on the metab- olism of invertebrates. To achieve this rate, the Daphnia have been kept in 10°C pond water for >60 . Your graphs and tables need to have numbers. Lab report of Daphnia magna heart rate. The percentage increase in average heart rate of D. magna after exposure to the respective concentrations of ammonium sulfate solution were calculated and a relationship between varying concentrations of ammonium sulfate concentration and the heart rate of D. magna was illustrated by plotting a graph using the respective data points obtained. 00 285. 5% caffeine . 6. Risk Assessment: In this experiment there are the following risks both for the human that carries out the experiment and the daphnia: ← The human that carries out the experiment should be cautious not to touch the lamp with wet hands since it uses electricity. 7. Daphnia have an open blood circulation. It was deducted that the alcohol and caffeine had a very notable effect on the heart, with alcohol slowing it down and caffeine speeding it up. Investigating factors affecting the heart rate of Daphnia.
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daphnia heart rate graph