telomerase activity in cancer cells

The can- In regard to this theory, many experiments have been conducted to detect telomerase in cancers . Accordingly, various classes of telomerase in … The exception is cancer cells possessing an active Alternative Lengthening of Telomeres (ALT) pathway. The cancer field started to take an interest in telomerase and telomere length approximately 7 years ago. Our data show that telomerase activity was inhibited only in differentiation-induced D2 cells but not in differentiation-induced melanoma and glioma cells. Telomerase is a good biomarker for cancer detection because most human cancers cells express high levels of it. Historical Background. This provides a selective growth advantage to many types of tumors. Sensitive detection of telomerase activity in cancer cells using portable pH meter as readout. Telomerase is an attractive molecular target toward which to direct cancer therapeutic agents because telomerase activity is present in most malignant cells but undetectable in most normal somatic cells. The enzymatic activity of telomerase is related but not equivalent to the expression of TERT, the catalytic. Short duplex RNA (short-interfering RNA or siRNA) has recently been shown to be an effective method for inhibiting the expression of a given gene in human cells. Telomerase activity is readily detected in most cancer biopsies, but not in premalignant lesions or in normal tissue samples with a few exceptions that include germ cells and hemopoietic stem cells. Telomerase is expressed in ~90% of human cancer cell lines and tumor specimens, whereas its enzymatic activity is not detectable in most human somatic cells, suggesting that telomerase represents a highly attractive target for selective cancer treatment. In the large majority of cancer cells, telomere length is maintained by telomerase. Measuring telomerase may be a way to detect cancer. Telomerase is a ribonucleoprotein DNA polymerase complex that maintains telomere length. 2018 Dec 15;121:153-158. doi: 10.1016/j.bios.2018.08.069. This is the rate-limiting step in telomerase activity. Telomerase activity is low or absent in normal cells, which have segments of repeat nucleotides called telomeres at the ends of their chromosomes that protect DNA stability during cell division, said first author Jian Hu, Ph.D., an instructor in MD Anderson's Department of Cancer Biology. Increased telomerase expression produces vulnerability of cancer cells, distinguishing them from normal cells in the body, although normal cells do also have some active telomerase. Recent studies also suggest that telomerase is implicated in tumor progression in unexpected ways. Recent studies also suggest that telomerase is implicated in tumor progression in unexpected ways. At present, little is known concerning resveratrol inducing apoptosis of skin cancer cells and its effect on the activity of telomerase. Telomerase action involves multiple steps including assembly of the telomerase complex, its intracellular trafficking and finally recruitment to telomeres. Therefore, various telomerase activity inhibitors have been developed to treat cancer but all failed due to side effects. Telomeres and telomerase in normal and cancer stem cells. If telomerase activity was to be turned off, then telomeres in cancer cells would shorten, just like they do in normal body cells. And if scientists can learn how to stop telomerase, they might be able to fight cancer by making cancer cells age and die. Moreover, differences in. It is believed that the telomerase just maintains the lengths of the telomeres. 2018 Dec 15;121:153-158. doi: 10.1016/j.bios.2018.08.069. A model for the differential inhibition of telomerase activity following induction of differentiation in different cancer cells will be presented. Mantell and Greider found that during early development, telomerase is highly active. We conducted the present study to determine the relationship between p53-dependent apoptosis and telomerase activity in ovarian cancer cells. The researchers also found that germline cells, such as cells found in the ovaries and testes of Xenopus, continued to produce telomerase. Telomerase is active in 85-95% of cancers ( 3, 4 ). Telomerase activity can be identified by its catalytic protein domain ( hTERT ). Measuring telomerase may be a way to detect cancer. A rare cell that escapes crisis almost universally does so by reactivating telomerase and this cell can now become a cancer cell with limitless potential to divide. Telomerase activity is low or absent in normal cells, which have segments of repeat nucleotides called telomeres at the ends of their chromosomes that protect DNA stability during cell division, said first author Jian Hu, Ph.D., an instructor in MD Anderson's Department of Cancer Biology. Increased telomerase expression produces vulnerability of cancer cells, distinguishing them from normal cells in the body, although normal cells do also have some active telomerase. Therefore, the removal of telomerase could be a possible treatment for cancer. It is associated with many cancer types. Telomere terminal transferase (telomerase) enzyme activity (not the identification of the genes encoding the components of telomerase) was discovered in 1985 in the single-cell organism Tetrahymena ().Almost a decade later, telomerase was described as an almost universal marker in advanced human cancers (), but it was not until 1997 that the catalytic protein component . Historical Background. Accordingly, various classes of telomerase in … In contrast, in cancer cells, such as bladder, uterine, stomach, esophageal, breast, thyroid, colorectal, laryngeal squamous cell, the activity of telomerase is prominent ( Ivancich et al., 2017, Patel et al., 2004 ). Telomeres maintain genomic integrity in normal cells, and their progressive shortening during successive cell divisions induces chromosomal instability. Therefore, various telomerase activity inhibitors have been developed to treat cancer but all failed due to side effects. Wild-type p53 genes were transducted to SK-OV-3 cells with a recombinant adenovirus that contained a wild-type p53 gene (AxCAp53). In cancer cells, surprisingly telomere length is not extended past the normal length. Telomerase enzyme activity has been detected in different origin of CD 34 + cells and has been significantly increased after the expansion of these cells in in vitro condition . Telomerase enzyme activity has been detected in different origin of CD 34 + cells and has been significantly increased after the expansion of these cells in in vitro condition . If telomerase activity was to be turned off, then telomeres in cancer cells would shorten, just like they do in normal body cells. Against telomerase activity, the reduction in telomere length is observed both in white blood cells and in hematopoietic stem cells in in vitro conditions [76,77]. Telomerase, the enzyme that repairs telomeres, exists in high quantities in developing organisms and in embryonic stem cells. Although telomerase was proposed to be expressed in tumor and not normal human tissue, evidence has accumulated that telomerase is expressed in a variety of normal tissues and that it is growth-regulated. Thus, telomere length and telomerase activity are crucial for cancer initiation and the survival of tumors. Telomeres and telomerase Telomerase Activity Is Correlated with Cell Proliferation in Normal Human Tissues Cells and Tumors. Telomerase activity, cell proliferation, and cancer Carol W. Greider* Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, 617 Hunterian Building, 725 N. Wolfe Street, Baltimore, MD 21205 Telomerase and telomere length have received a lot of recent attention from the biomedical research community. 2. In the large majority of cancer cells, telomere length is maintained by telomerase. Telomerase activity, cell proliferation, and cancer. Telomerase is expressed in ~90% of human cancer cell lines and tumor specimens, whereas its enzymatic activity is not detectable in most human somatic cells, suggesting that telomerase represents a highly attractive target for selective cancer treatment. Telomeres were active throughout embryogenesis and oogenesis in Xenopus egg cells. Telomerase and telomere length have received a lot of recent attention from the biomedical research community. In the present study, resveratrol intervention was used on cultured human A431 epidermoid carcinoma cells, and its value-added influence on A431 cell apoptosis and telomerase activity was observed. Telomerase, an enzyme responsible for the maintenance of telomere, is silent in most normal somatic cells but activated in 90% of cancer cells, making it an excellent target for cancer therapy. IC . Some cells have higher amounts of telomerase activity than do others. This was originally . It is believed that the telomerase just maintains the lengths of the telomeres. In theory, a lack of telomerase would retard the growth of tumors by causing continually dividing cells to lose their telomeres and to die before they did any damage. Active telomerase is essential for stem cells and most cancers to maintain telomeres. Telomerase, an enzyme responsible for the maintenance of telomere, is silent in most normal somatic cells but activated in 90% of cancer cells, making it an excellent target for cancer therapy. Epub 2018 Aug 30. ALT, which is the ability of cancer cells to extend telomeres in the absence of telomerase, is based on homologous recombination using telomeric DNA as a matrix ( 45 ). Telomerase activity may, therefore, be a useful biomarker for diagnosis of malignancies and a target for inactivation in chemotherapy or gene therapy. Biosens Bioelectron. Against telomerase activity, the reduction in telomere length is observed both in white blood cells and in hematopoietic stem cells in in vitro conditions [76,77]. In this model, telomerase activation occurs after the selection of mutations that stimulate abnormal cell proliferation, and, thus, telomerase activation may be a relatively late event in tumor initiation that allows for genome stability after the acquisition of cancer-associated mutations. The complex comprises the protein telomerase reverse transcriptase (TERT, or hTERT in humans) and a catalytic telomerase RNA component (TERC) 4). Telomere terminal transferase (telomerase) enzyme activity (not the identification of the genes encoding the components of telomerase) was discovered in 1985 in the single-cell organism Tetrahymena ().Almost a decade later, telomerase was described as an almost universal marker in advanced human cancers (), but it was not until 1997 that the catalytic protein component . Telomerase is the cellular ribonucleoprotein enzyme complex that catalyzes the extension of telomeric DNA in eukaryotic organisms. Telomerase has been detected in human cancer cells and is found to be 10-20 times more active than in normal body cells.

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